Brain Capsule Qualitative and Quantitative Analysis.docVIP

 PAGE \* MERGEFORMAT 10 Brain Capsule Qualitative and Quantitative Analysis Author: Yu-Xin, Li Ling, LI Yu-feng, YANG Wen-yu [Keywords:] Brain Capsule Abstract: The purpose of the brain Capsule Salvia, Chuanxiong, thin-layer chromatography for qualitative identification of puerarin and determination of puerarin in cerebral capsule content. Methods The thin-layer chromatography (TLC) on these three herbs to taste identification, mobile phase, respectively benzene ethyl acetate (19:1), n-hexane ethyl acetate (9:1), chloroform methanol water (78:35 : 10) in the lower fluid; using RP-HPLC determination of puerarin, Diamonsil C18 column (5 μ m, 150 mm * 4.6 mm), methanol-0.015% phosphoric acid (seventy-seven past eleven p.m.) as mobile phase a flow rate of 1.2 ml / min, column temperature 35 ℃ , detection wavelength 249 nm, measured. The results of HPLC determination of linear range of 0.02 ~ 0.12 μ g, the correlation coefficient r = 0.999 9; Capsule puerarin, the average recovery rate of 98.53%, RSD = 1.08%, n = 6; precision (RSD amp;lt;2%) Good . Conclusion The method is simple, rapid, accurate and applicable to the brain capsule in the identification and characterization of the main ingredients of puerarin HPLC. Keywords: puerarin; brain capsule; thin-layer chromatography; high-performance liquid chromatography Brain capsule by the puerarin, Chuanxiong, red sage root and other medicinal preparations composed of Chinese herbal compound, with replenishing liver and kidney, replenishes the marrow, Huoxuetongmai effect. Primarily for the treatment of cerebral arteriosclerosis liver and kidney essence deficiency and blood stasis card, Zheng Jian dizziness, headache, Shenpi forgetfulness, chest tightness or pain, dry mouth head Shibuya, Yaoxisuanruan, virtual annoying little sleep soundly and so on. In order to control the quality of this product, I used TLC method formulations contained Chuanxiong, Salvia, Pueraria, etc. 3 flavor medicine TLC carried out experime