Chinese cobra venom on human salivary adenoid cystic carcinoma cell line of small cell cycle and cell apoptosis.docVIP

 PAGE \* MERGEFORMAT 15 Chinese cobra venom on human salivary adenoid cystic carcinoma cell line of small cell cycle and cell apoptosis Authors: LIU Cheng-jun, Wei Shi-Xiu, Li Mu-Yan, Wu, Jia-Quan Li [Abstract] Objective To study the Chinese cobra venom (Chinese cobra venom) on cultured human small salivary adenoid cystic carcinoma (NACC) cell lines induced apoptosis. Methods ATP - Bioluminescence chemotherapy drug sensitivity test method (ATP-TCA) inhibit the growth of cells was determined by the percentage of inverted phase contrast microscope, HE staining cells were observed morphological changes, flow cytometry cell cycle and cell apoptosis. ATP-TCA results of experiments showed that cobra venom on the NACC in vitro significantly inhibited cell growth, and directly proportional to the concentration and action time; morphology observed after treatment of the NACC cytoplasmic vacuoles, nuclear pyknosis, nuclear chromatin aggregation, etc. morphological change phenomenon; flow cytometry found that cobra venom NACC block cell growth in the G0/G1 cell cycle phase, to detect apoptosis in the experimental group was significantly higher than that. Conclusion Chinese cobra venom NACC cells can inhibit proliferation and induce apoptosis NACC. [Keywords:] China cobra venom NACC cell line cell cycle Apoptosis Abstract: Objective To study on the effect of Chinese cobra venom-induced apoptosis in Noovel Human palatal salivary gland cells line. MethodsThe adenosine triphospate (ATP)-bioluminescence tumor chemosensitivity assay (ATP-TCA) was used to examine the growth of NACC cells treated with Chinese cobra venom in different concentration.Cell apoptosis was observed by flow cytometry and cell morphology. ResultsThe experiment showed that cobra venom could obviously inhibit the growth of NACC cell in a concentration and time-dependent manner.The changes in morphology of NACC Cell were observed by HE staining. The flow cytometry analysis showed that apoptosis and inhib