Chloride channel blocker on human trabecular meshwork cell proliferation.docVIP

 PAGE \* MERGEFORMAT 10 Chloride channel blocker on human trabecular meshwork cell proliferation Study: Dan Wang Jianguo Liu Ding Li Chao will [Abstract] Objective To investigate the chloride channel blocker 5-nitro-2 (3 amphetamine) benzoic acid (5 nitro 2 (3 phenyl propylamino) benzoic acid, NPPB) on cultured human trabecular meshwork cells. Methods RT PCR detected in cultured human trabecular meshwork cells ClC 3 mRNA expression, the role of different concentrations of NPPB cultured human trabecular meshwork cells, cell counting trypan blue cell viability assay, tetrazolium blue method (MTT proliferation was detected. Results in cultured human trabecular meshwork cells exist ClC 3 mRNA expression, the role of different concentrations of NPPB trabecular meshwork cells after no significant difference in survival, in a certain concentration range (50 ~ 100 mol / L, NPPB can make cultured human trabecular meshwork cells decreased proliferation, in which 100 mol / L NPPB the strongest effect. Results Conclusion certain concentration NPPB reduces the in vitro proliferation of human trabecular meshwork cells. [Keywords:] 5-nitro-2 (3 amphetamine) benzoic acid (NPPB, trabecular cells, glaucoma, RT PCR, MTT method [Abstract] Objective To research the effect of chloride channel inhibitor (5 nitro 2 (3 phenyl propylamino) benzoic acid, NPPB) on cultured human trabecular meshwork cells in vitro.Methods The mRNA expression of ClC 3 in cultured human trabecular meshwork cells was detected in vitro with RT PCR.The cultured human trabecular meshwork cells in vitro were treated with different density of NPPB to detect the cell survival rate with trypan blue stain and cytometry, and to measure the cell proliferation with MTT.Results ClC 3 mRNA expressed in cultured human trabecular meshwork cells in vitro.During the density from 50 ~ 100 mol / L, the cell survival rate had no significant difference, but the cell proliferation decreased.100 mol / L NPPB had the mos