Chronic hypoxia and carbon dioxide on mitochondrial function in mouse brain.docVIP

 PAGE \* MERGEFORMAT 15 Chronic hypoxia and carbon dioxide on mitochondrial function in mouse brain Of: Wu Bin, Jin Lu, Wang same, Ren Huiming [Abstract] Objective?: To investigate the chronic hypoxia and brain mitochondrial function in mice. METHODS: Male C57BL / 6 mice 30 were randomly divided into 2 groups: A group (15 rats exposed to hypoxia group) and B group (normal control group, 15). A group raised in the chamber, the chamber oxygen concentration of 9% to 11%, carbon dioxide concentration of 5% -6% daily 8 h ~ week 6 d, 4 weeks , like the rest of the time with the B group, living in normal environments. measuring brain tissue ATP, ADP, AMP concentration; mitochondrial membrane potential, COX and activity. Results: mice exposed to hypoxia and brain ATP, AMP was significantly lower in the control group (P lt;0.01), mitochondrial morphological changes, the brain mitochondrial membrane potential is low, COX and was inhibited (P lt;0.01), brain tissue SOD, GSH activity decreased (P lt;0.01). Conclusions: Chronic low oxygen high carbon dioxide can lead to mouse brain mitochondrial function was abnormal, which may be related with oxidative stress. [Keywords] mitochondria; hypoxia, hypercapnia; oxidative stress response; mice Abstract:? Objective: To explore the influence of chronic hypoxic hypercapnia on the mitochon-dria in the cerebrum of the mice.? Methods:? Thirty male C57BL / 6 mice were randomly divided into two groups: the hypoxic hypercapnia 4-week experiment group (A group) and normal control group (B group). The experiment group was exposed to an atmosphere containing 9% ~ 11% O2 and 5% ~ 6% CO2 8 hours a day, 6 days a week for 4 weeks. The rest time A group lived in the room as B group did. The concentration of ATP, ADP and AMP was measured by HPLC. The changes of the ultramicrostructures in the mitochondria of the cerebrum were observed under the transmission electron microscope.The membrane potential of the mitochondria was observe