Cigarette smoke extract induced effect of bone marrow micronucleus.doc

 PAGE \* MERGEFORMAT 5 Cigarette smoke extract induced effect of bone marrow micronucleus [Keywords:] cigarette smoke extract the role of bone marrow cell micronucleus Our previous studies have demonstrated that cigarette smoke dimethyl sulfoxide (DMSO) extracts can be induced chromosome damage in germ cells [1], while mice, superoxide dismutase (SOD), malondialdehyde (MDA) levels increased [2], that cigarette smoke extract could increase peroxidation in mice, and thus lead to germ cell mutations, their health of future generations to bring possible threats. cigarette smoke extract DMSO may also be increased peroxidation role in the genetic material of cells and cause their damage. Therefore, we DMSO cigarette smoke extract on mouse bone marrow cells induced the production of micronuclei carried out studies, reports as follows. 1 Materials and methods 1.1 Preparation of cigarette smoke extract Removed with a commercially available filter-tipped cigarettes, a brand of light to flow the negative pressure smoking, tobacco control regulating flow rate of about 10 min for each cigarette burn in the finish. With 10% DMSO extract as to the weighing method to measure extract was extract the contents, diluted with distilled water for use. 1.2 Animal treatment Kunming mice, provided by the Baotou Medical College animal house, male, weight 21 + -1 g, age 90 d. Rats in the laboratory breeding of animals 2 weeks after the election health were randomly divided into three observation group (A, B, C), 1 a blank control group and a solvent control group, n = 10 animals. A, B, C observed in the daily morning group 1 according to the weight (mg / kg) were injected 50.0,25.0 , 12.5 mg of cigarette smoke extract (dissolved in 0.3 mL 10% DMSO), continuous exposure to 5 d, animals were killed the afternoon of the first 5 d. solvent control method according to the observation group were injected intraperitoneally 10% DMSO, each 0.3 mL; blank exposed control mice a