Compound on the HPLC determination of the film Yiganling content of silybin.docVIP

 PAGE \* MERGEFORMAT 10 Compound on the HPLC determination of the film Yiganling content of silybin Study: Ancient sea front, Zhang Amanda, often by Rong, Fuxin Tong, Guo Zhu [Abstract] Objective To establish the compound Yiganling silibinin film method for the determination. Methods HPLC, mobile phase: methanol - water - acetic acid (48:52:1), detection wavelength: 286 nm. The result of water silybin in the range 0.238 0 ~ 2.380 μg good linearity, the regression equation: Y = 2.146 × 106X +1.818 × 103, r = 0.999 9, the average recovery was 98.17%, RSD = 1.3%. Conclusion The method is simple, accurate, reproducible and can be used as compound Yiganling the quality control methods. [Key Words] high performance liquid chromatography, compound Yiganling films, silibinin Determination of Silybin in Fufang Yiganling Tablet by HPLC GU Hai-feng, ZHANG Xiao-qian, CHANG Zeng-rong, et al Beijing Institute for Drug Control, Beijing 100035, China Abstract: Objective To establish a method for the determination of silybin in Fufang Yiganling tablet. Methods RP-HPLC was used and the mobile phase was consisted of methanol-water-glacial acetic acid (48:52:1). The detection wavelength was 286 nm. Results The calibration curve of Silybin was linear in the range of 0.238 0 ~ 2.380 μg, linear equation: Y = 2.146 × 106X +1.818 × 103, r = 0.999 9, with average recovery of 98.17% (RSD = 1.3%). Conclusions The method is simple, accurate and with good reproducibility. It can be used to control the quality of silybin in Fufang Yiganling tablet. Keywords:: HPLC, Fufang Yiganling tablet, Silybin compound Yiganling film by the silymarin, Nuts alcohol extract processed with Yigan ney, detoxification Qushi and so on, for the clinical treatment of acute and chronic hepatitis commonly used drugs. which silymarin is the main component silybin, silibinin has two isomers: silybin A (silybin A), silybin B (silybin B) [1]. Original quality standard closing contained in lt;lt;The Ministr