CpG N ODN on CpG S ODN-induced hPBMC inhibition of the release of TNF α.docVIP

 PAGE \* MERGEFORMAT 10 CpG N ODN on CpG S ODN-induced hPBMC inhibition of the release of TNF α Author: Jiang Wei Wang Liangxi for spare pairs Jiang Ding 【Abstract】 Objective To inhibitory CpG ODN 208 (CpG  N ODN) on the irritating CpG ODN 1826 (CpG  S ODN)-induced monocyte / macrophage secretion of TNF  α influence to achieve the prevention and treatment through the antagonistic bacterial DNA pus to provide experimental basis for the purpose of thyrotoxicosis. Methods pre-screened out of this room right CpG  S ODN-activated RAW 264.7 cells inhibited the CpG  N ODN, observe their CpG  S ODN-induced hPBMC the impact of the release of TNF  α; application of flow cytometry CpG  N ODN on CpG  S ODN and hPBMC surface binding and internalization effects. The results of the concentration ratio of 1:1 CpG  N ODN for stimulating CpG  S ODN-induced hPBMC inhibit secretion of TNF  α, and showed a certain time-effect relationship; CpG  N ODN on CpG  S ODN in the combination of cell surface hPBMC and internalization inhibited. Conclusion CpG  N ODN on CpG  S ODN activation hPBMC inhibited, this effect may be related to the impact hPBMC surface binding and internalization of CpG  S ODN on. Keywords: Sepsis inhibitory CpG ODN stimulation CpG ODN TNF  α internalization of surface binding ABSTRACT Objective To study the influence of CpG ODN 208 (CpG  N ODN) on TNF  α release from hPBMC induced by CpG  S ODN 1826 (CpG  S ODN). Methods CpG  N ODN, selected by the previous work in our lab, can inhibit TNF  α release from RAW264.7 induced by CpG  S ODN. The influence of CpG  N ODN on TNF  α release from hPBMC induced by CpG  S ODN was measured by ELISA. The effects of CpG  N ODN on the surface binding and internalization of CpG  S ODN were tested by flow cytometry. Results CpG  N ODN could inhibit TNF  α release from hPBMC induced by CpG  S ODN at the concentration ratio 1:1 in a time  dependent manner . CpG  N ODN coul