Cubeb supercritical extract and Pharmacodynamic Study of Screening Process.docVIP

 PAGE \* MERGEFORMAT 6 Cubeb supercritical extract and Pharmacodynamic Study of Screening Process Author: Wang Tzu-yuan, HE Ze-min, JIANG Xiao-Han [Abstract] Objective To screen the supercritical CO2 fluid extraction of volatile components Cubeb the best technology, and to study the efficacy extracts. Methods orthogonal experimental design, in order to extract yield as an indicator to study extraction pressure, extraction temperature, pressure and analytical analysis temperature four factors; experiments in vitro antibacterial and anti-allergic sensitized guinea pig ileum muscle contraction experiments , acetic acid writhing test, mice the number of activities affect the test independently extract the main efficacy study. Results The optimum conditions for extraction pressure extraction process 25MPa, extraction temperature 40 ℃ , parsing the pressure 8 MPa, analytical temperature 50 ℃ ; extract has significant antibacterial, anti-allergic, analgesic, sedative and other pharmacological effects. Conclusion Supercritical CO2 Fluid Extraction Technology for Cubeb volatile components extraction method is simple and feasible for industrial production. Cubeb supercritical fluid extract has a wide range of pharmacological effects, the value of a larger research and development. [Keywords:] Cubeb; supercritical carbon dioxide extraction; pharmacological effects Abstract: ObjectiveTo study the optimal technology of supercritical-CO2 fluid extraction (SFE-CO2) for Litsea cubeba (Lour.) Pres.and its pharmacodynamic effect.MethodsThe yield of extract was used as index to evaluate the technologies based on orthogonal design, in which 4 factors considered were extraction temperature and pressure, separation temperature and pressure; the animal models of bacteriostasis, anti-anaphylaxis, analgesia, conscious-sedation were used to study the function of the extract. ResultsThe optimum extraction conditions was extraction pressure 25MPa, temperature 40 ℃ , separation pressur