Cytometric bead array assay IFN-γ-induced Th1-Th2 cytokine expression in Jurkat cells,.docVIP

 PAGE \* MERGEFORMAT 13 Cytometric bead array assay IFN-γ-induced Th1/Th2 cytokine expression in Jurkat cells, Abstract This study aims to explore the cytometric bead array assay Th1/Th2 cytokines IFN-γ expression and T lymphoblastic leukemia treatment used in clinical value. With different concentrations of IFN-γ-induced Jurkat cells cultured for 48 hours, using cytometric bead array assay supernatant IL-2, IL-6, TNF-α and IFN-γ expression, using flow cytometry membrane IL-2 receptor (CD25) expression. The results showed that: IFN-γ-induced Jurkat cells, IL-2 and TNF-α expression in a dose-dependent increase was not detected IL-6 expression, CD25 expression increased significantly. Conclusion: Jurkat cells can be induced by IFN-γ high expression of Th1 cytokines and CD25, cytometric bead array method can accurately and objectively reflect the expression of Th1/Th2 cytokines dynamics. 【Key Words】 T-lymphocyte leukemia Detection of Expression of Th1/Th2 Cytokines in Jurkat cell Treated with γ-Interferon by Cytometric Bead Array Abstract In order to explore the value of γ-interferon (IFN-γ) in therapy of T lymphoid leukemia and role of cytometric bead array (CBA) method in detection of Th1/Th2 cytokines expression, the Jurkat cells were cultured for 48 hours in different concentration of IFN-γ, the expressions of IL-2, IL-6, INF-γ and TNF-α in culture supernatants were assayed by CBA method; CD25 expression was assayed by flow cytometry.The results showed that the expressions of IL -2, TNF-α were enhanced in INF-γ dose-dependent way, and the expression of CD25 was also enhanced, but there was no expression of IL-6. It is concluded that Jurkat cells induced by IFN-γ were able to express high-level of Th1 cytokine and IL-2 membrane receptor (CD25), and the CBA method can be used to exactly evaluate the dynamic change of Th1/Th2 cytokines. Keywords: cytometric bead array; Th1/Th2 cytokine; IFN-γ; T lymphoid leukemia Cytomet