DC co-cultured with CIK cells on the cytotoxic activity of liver cancer.docVIP

 PAGE \* MERGEFORMAT 10 DC co-cultured with CIK cells on the cytotoxic activity of liver cancer Authors: Bao-An Chen, Lehman, SUN Zai-Yang, Cui-Ping Li, Gao Chong, Yun-Yu Sun Abstract The purpose of this study is to observe the cytokine-induced killer (CIK) and the homologous dendritic cells (DC) were cultured in DC-CIK cells proliferation, phenotypic changes and its impact on liver cancer cells cytotoxic effects. Collected from healthy donors of peripheral blood mononuclear cells (MNC), placed in 37 ℃, 5% CO2 incubator cultured for 2 hours, to collect non-adherent cells used to induce cultured CIK cells, adherent cells were differentiated into mature DC, will be mature DC and CIK cells, according to the ratio of 1:5 mixed culture 3 days, using MTT assay DC-CIK cells co-cultured with anti-hepatoma cell line SMMC-7721 activity. The results showed that: DC and CIK cells were cultured, DC-CIK cells group proliferation and cytotoxicity of CIK cells, higher than the pure. Conclusion: DC co-cultured with CIK cells are a kind of proliferation and cytotoxic activity of CIK cells were higher than the immune competent cells. Keywords: hepatocellular carcinoma cells Killing Activity in DC and CIK Co-culture against Hepatocar-cinoma Cells Abstract This study was aimed to investigate the proliferation activities and phenotype changes of DC, CIK and DC-CIK, and their cytotoxicity against hepatocarcinoma cells in co-culture of DC with CIK. Peripheral blood mononuclear cells (PBMNC) were isolated from heathy adult donors . After incubation of PBMNC for 2 hours, DCs were induced from adherent cells by some cytokines and CIKs were generated from non-adherent cells. Mature DCs were harvested after incubation for 9 days, and then were co-cultured with CIK at ratio of 1 : 5 for 3 days. The cytotoxicity activity against SMMC-7721 hepatocellular carcinoma cell line was detected by MTT assay. The results showed that CIK cells were able to lyse SMMC-7