DEAE-Sephadex A-50 purified immunoglobulin F (ab ) 2 of.docVIP

 PAGE \* MERGEFORMAT 6 DEAE-Sephadex A-50 purified immunoglobulin F (ab ‘) 2 of [Abstract] Objective DEAE-Sephadex A-50 purification, an improvement in tetanus anti-toxin immune globulin F (ab ‘) 2 purity. Methods tetanus immunization with pepsin digestion of plasma, ammonium sulfate precipitation, alum purified immunoglobulin F (ab ‘) 2, after DEAE-Sephadex A-50 purified and tested. Results the above method to digest 2h, 95% or more of the IgG hydrolysis as F (ab ‘) 2, after DEAE-Sephadex A-50 after purification of tetanus anti-toxin immune globulin F (ab’) 2 content of up to 75% or more than activity increased significantly. Conclusion DEAE-Sephadex A-50 purified tetanus anti-toxin immune globulin F (ab ‘) 2 is a more desirable method. [Keywords:] Tetanus Antitoxin F (ab ‘) 2 purified DEAE-Sephadex A-50 [Abstract] Objectives In order to improve the purity of TAT immunoglobulin F (ab ‘) 2, we purified it by DEAE-Sephadex A-50.Methods The immunoglobulin F (ab’) 2, produced from the tetanus immune plasma which was digested by pepsin, separated by ammonium sulphate, purified by alum, was purified by DEAE-Sephadex A-50 and was carried on all reqired tests.Results After digestion for 2 hours in mentioned situation, over 95% IgG hydrolysis F (ab ‘) 2 , and after purification by DEAE-Sephadex A-50, the content of F (ab ‘) 2 reached over 75%, and the specific activity increased greatly.Conclusion Purification of TAT immunoglobulin F (ab’) 2 by DEAE-Sephadex A - 50 is an ideal method. [Keywords:] tetanus antitoxin (TAT); F (ab ‘) 2; purification; DEAE-Sephadex A-50 In recent years, technology has been widely used in chromatography separation and purification of biological polymers, it was reported that a number of Biochemical Factory at home and abroad using chromatographic techniques plasma protein separation, purification of the vaccine [1]. Tetanus antitoxin salting out method currently used products exist hybrid protein content high, especially after the pepsi