Death-associated protein kinase gene promoter methylation specific PCR and its preliminary application.docVIP

 PAGE \* MERGEFORMAT 13 Death-associated protein kinase gene promoter methylation specific PCR and its preliminary application Of: Wang Yali Yao Dongming Lin, Jiang Qian Jun Qian Zhen Xu Wenrong Xiaogao Fei Wu Chaoyang Jiang Pengcheng Zhu Zhaohui [Abstract] Objective: To establish a methylation specific PCR (MSP detection of death-associated protein kinase (DAPK gene promoter methylation. Methods: Design for the DAPK gene promoter MSP primers, the establishment of MSP for methylation-positive detection system template for testing, evaluation of its specificity, reproducibility and sensitivity, and by DNA sequencing. Results: The established method of methylation can only amplify the MSP methylation positive template, you can not amplify unmethylated positive templates and template DNA is not curing treatment, different gradient of dilution of the positive template for methylation detection sensitivity of up to 2% of its maximum, at different times MSP methylation test results were consistent. Conclusion: The established DAPK gene promoter methylation in MSP detection method has good specificity, sensitivity and repeatability, can be used to detect the bulk tumor samples. [Keywords:] death-associated protein kinase; methylation specific PCR; methylation [Abstract] Objective: To establish the methylation specific PCR (MSP) for the detection of promoter methylation of death associated protein kinase (DAPK) gene.Methods: The primers of MSP aiming at the DAPK promoter were designed.MSP was established to detect the methylation positive DNA templates.The specificity, sensibility and repeatability were evaluated. Results: The established methylated MSP could only amplify the methylation positive DNA template, but not the unmethylated DNA template and unmodified DNA template.In a series of dilution, methylated MSP could detect 2% methylation positive DNA templates.The amplified results of same sample were identical on different time. Conclusion: The methyla