Derivatization RP-HPLC determination of Huangyangning Tablets Cyclovirobuxine D content of.docVIP

 PAGE \* MERGEFORMAT 10 Derivatization RP-HPLC determination of Huangyangning Tablets Cyclovirobuxine D content of [Abstract] Objective To establish a reversed-phase high performance liquid chromatography (RP-HPLC) determination of Huangyangning Tablets Cyclovirobuxine D content. Methods cyclovirobuxine D with phenyl isocyanate reaction, measured using RP-HPLC analytical column was ODS-C18 column; mobile phase was methanol - water (84:16); detection wavelength: 234 nm. The results derived from reaction at the 23.03 ~ 53.73 μ g * ml-1 concentration range of good linearity, r = 0.999 8 (n = 5), the mean recovery rate of 96.1% and good reproducibility. Conclusion The method is accurate, reliable, specific and can be used Huangyangning film Cyclovirobuxine D, quality control. [Keywords:] Huang Yang Ning-chip Cyclovirobuxine D phenyl isocyanate derived from the role of RP-HPLC Abstract: ObjectiveTo develop an RP-HPLC method for quantitative determination of Cyclovirobuxine D in HuangYangNing tablets. MethodsCyclovirobuxine D reacted with a derivative reagent phenyl isocyanate in chloroform.The analysis was carried out on C18 column, the mobile phase was methanol-water (86: 14), and the detection wavelength was at 234 nm.ResultsGood linearity was obtained from 23.03 μ g * ml-1 to 53.73 μ g * ml-1 of cyclovirobuxine D derivatives with a correlation coefficient of 0.999 8.The average recovery was 96.1%, RSD = 1.5%. ConclusionThe method is accurate, reliable and specific and can be used for the quality control of Huangyangning tablets. Keywords:: Huangyangning Tablets; Cyclovirobuxine D; Phenylisocyanate; RP-HPLC; Derivatization Huang Yang Ning film mainly composed of Cyclovirobuxine D, Cyclovirobuxine D is from plants of lobular boxwood Buxus Buxus minrophlla Sieb. Et Zucc. Var.SincaRehd. Et Wils. And its refined extract derived from the same plant, mainly used in the treatment of coronary heart disease and angina pectoris. Because this strain extracted from