Determination by HPLC Millettia Ointment formononetin content.docVIP

 PAGE \* MERGEFORMAT 9 Determination by HPLC Millettia Ointment formononetin content [Abstract] Objective: A high performance liquid chromatography Millettia active ingredient formononetin content. Methods: According to high-performance liquid chromatography to 18 alkyl silane bonded silica gel as a filler, acetonitrile - water (33:67) as mobile phase, detection wavelength 250nm, column temperature 35 ℃ , flow 1.0ml/min ; sample volume 10μ l, theoretical plate number according to formononetin calculation should not be less than the 5000 peak. Results: The preparation of the auxiliary interference-free determination of the main drug. Formononetin in the sample volume 0.01025μ g ~ 0.5125μ g (injection volume 10μ l) showed good linear relationship within the framework. Precision RSD of 0.2% for the test product was stable solution in 10 hours. Measured average recovery 98.73%, RSD 0.24%, indicating that recovery was in line with relevant regulations. Conclusion: The method is simple, rapid, accurate, specific and strong. [Keywords:] Millettia; high-performance liquid chromatography; determination; related substances Millettia paste is mainly used for blood and blood circulation, Shujin vibrant, and cure waist and knee pain, numbness, rheumatism, paralysis, amenorrhea, dysmenorrhea, irregular menstruation, etc. [1]. In this study, high-performance liquid chromatography in which an active ingredient formononetin content, method is simple, rapid, accurate, specific and strong. An instrument, test drugs and experimental samples DIONEX HPLC Liquid Chromatography ULTIMAT 3000; Balance AG135 and BS210S (Mettler - Toledo); UV sub-photometer UV-500 (British thermal); acetonitrile HPLC pure, water re-distilled water, the rest of the reagents were of analytical grade. Formononetin reference substance (Lot No. :111703-200501) for Chinese medicines and biological products available. 2 Methods and Results 2.1 Chromatographic conditions and system suitability test To 18