Detection of Staphylococcus aureus biofilm and the relationship with drug resistance.docVIP

 PAGE \* MERGEFORMAT 14 Detection of Staphylococcus aureus biofilm and the relationship with drug resistance Of: Zhang Liping, Li Qing, Gulina Yiming, Li Lingling, Tian Gang [Abstract] Objective To investigate the biofilm formation of Staphylococcus aureus in clinical specimens of the situation, research whether the resistance of Staphylococcus aureus biofilm difference, to help the clinical use of antimicrobial drugs based on susceptibility results, timely and effective control of staphylococcal hospital infection. Methods Congo red, micro plate assay of biofilm formation, observation of glucose, ethanol and sodium chloride on biofilm formation; of Staphylococcus aureus isolated from clinical samples for identification and susceptibility testing. The results detected by Congo red saprophytic Staphylococcus aureus Staphylococcus aureus (S. aureus) ?p positive rate of Staphylococcus epidermidis biofilm was 10.0%, 16.7%, 40.0%, biofilm semi-quantitative method: The positive rate of Staphylococcus epidermidis biofilm TSB, TSB 0.25% Glu, TSB 2% Alc , TSB 4% Alc, TSB 2% NaCl, TSB 4% NaCl were 13.5% and 34.6%, 33.3%, 29.2%, 19.4%, 32.1%, and 0.25% Glu, TSB 2% Alc, TSB 4% NaCl as optimal concentration of drug susceptibility results, resulting in biofilm resistant Staphylococcus aureus to penicillin, erythromycin and cotrimoxazole increasing trend of drug resistance, resistance rates were: 86.43%, 82.44%, 78.35%. Conclusion The majority of clinical specimens can produce biofilm Staphylococcus aureus, glucose, ethanol, sodium chloride can promote biofilm formation of Staphylococcus clinical resistance to antimicrobial agents commonly used in serious infections caused them available vancomycin. [Keywords:] resistant Staphylococcus aureus biofilm susceptibility testing [Abstract] Objective To study the formation of staphylococcal biofilm and the difference of drug resistance between Staphylococcus with or without biofilm, helping to choose the antibiotic acc