Determination of 3 kinds of Zushima phenylpropanoids of the Content.docVIP

 PAGE \* MERGEFORMAT 8 Determination of 3 kinds of Zushima phenylpropanoids of the Content Study: Among static, single march, Liu Jia, Hao, Di Liu-qing [Abstract] Objective To establish the HPLC method for simultaneous determination of Daphnetin Zushima ,7-OH coumarin, syringin content. Methods using YMC-Pack ODS column (250 mm × 4.6 mm, 5 μm), to methanol -0.5% acetic acid - water (8 ︰ 19 ︰ 1) as mobile phase, dual-wavelength detection, λ1 = 327 nm, λ2 = 266 nm, flow rate 1.0 mL / min, column temperature of 30 ℃. the results of three components were can be baseline separated, the average recovery of each component in between 98.41% ~ 100.18%. Conclusion is simple, reliable, reproducible, and can be used as the method for quality control of medicines. [Keywords:] Zushima, high performance liquid chromatography, Daphnetin ,7-OH coumarin, syringin, determination Abstract: Objective To establish a HPLC method for the determination of daphnetin, 7-OH coumarin and syringing in Zushima. Methods The separation was performed on YMC-Pack ODS (4.6 mm × 250 mm, 5 μm) column with a mobile phase consisting of methanol-0.5% acetic acid-water (19 ︰ 8 ︰ 1) as gradient elution at the flow rate of 1.0 mL / min. The dual detective wavelength was set at 327 nm and 266 nm. The column temperature was 30 ℃. Results Baseline separation was achieved for the analysis of the three index components. The average recoveries were 98.4% ~ 100.2%. Conclusion This method is convenient, rapid and accurate. It can be used for quality control in production of Zushima. Keywords:: Zushima, HPLC, daphnetin ,7-OH coumarin, syringin, content determination Ma is the founder of plant Huang Rui Hong Thymelaeaceae Daphne giraldii Nitsche, Shaanxi and Gansu Daphne Daphne tangutica Maxim or concave leaves Daphne Daphne retusa Hemsl dry bark and root bark, spring and autumn harvest stripping, drying, is a folk herbal medicine. In order to good way of quality control Zushima medicine, this st