Determination of artemisinin content in Artemisia annua plant improvement and quality assessment.docVIP

 PAGE \* MERGEFORMAT 12 Determination of artemisinin content in Artemisia annua plant improvement and quality assessment [Abstract] Objective To establish a quick and accurate determination of artemisinin content in Artemisia annua method to guide the acquisition of raw materials of artemisinin and artemisinin production process quality control. Improvement of UV spectrophotometry, detection wavelength 292 nm, artemisinin content of the formula: M (mg / g) = (A sample * V * r * 2) / (A standard * 3). The results of artemisinin reference substance in the 0.02 ~ 0.14 mg / ml within the framework of a good linear relationship, r = 0.999 8 (n = 7), the average recovery was 99.81% (n = 7). Youyang County of Chongqing were seven samples from different areas of artemisinin content in Artemisia annua range of 6.71 ~ 10.19 mg / g. Determination results with the traditional methods of analysis no significant difference. Conclusion Improved ultraviolet spectrophotometry for determination of artemisinin is simple, good linearity and repeatability, you can replace the traditional methods of analysis of artemisinin production process as the determination of the new methods. [Keywords:] Artemisia annua artemisinin-based quality control of UV spectrophotometry Abstract: ObjectiveTo establish a fast and precise method to determine the arteannuin content in Artemisia annua L., which can give guidance to buying arteannuin raw material and quality control in Artemisia annua L.production.MethodsModified Ultraviolet spectrophotometry was adopted, the detection wavelength was 292 nm, and the calculation formula about arteannuin was M (mg / g) = (Asample * V * r * 2) / (Astandard * 3). Resultscontrol article had a good linear relationship in the range of 0.02 ~ 0.14 mg / ml, r = 0.999 8, the average recovery was 98.81% (n = 7). Among seven different areas in Youyang county in Chongqing, the extent of arteannuin content in Artemisia annua L. sample was 6.71 ~ 10.19 mg / g. The resul