Determination of atmospheric particulate matter in the heterocyclic amines.docVIP

 PAGE \* MERGEFORMAT 16 Determination of atmospheric particulate matter in the heterocyclic amines Of: Dong Xueling  Shao-peng Liu, manganese and high [Abstract] established the atmospheric particulate matter in the heterocyclic amines by HPLC method. Using ODS C18 column (250 mm × 4.6 mm, 5 μm), acetonitrile and 0.01 mol / L triethylamine buffer (pH 4.0) as mobile phase, linear gradient elution, flow rate 1.0 mL / min, column temperature 30 ℃, UV detection wavelength of 263 nm, and fluorescence excitation and emission wavelengths optimized conditions for the realization of the 6 baseline separation of heterocyclic amines and 4 miscellaneous cyclic amines of high sensitivity fluorescence detection. The method of detection of fluorescence and UV detection limits were 0.0018 ~ 0.0084 mg / L and 0.093 ~ 0.609 mg / L (S / N = 3), the correlation coefficient of 0.9920 ~ 0.9999 room, RSD less than 5.9% and the average recovery was 111.6% 75.3% recovery of the relative standard deviation was 1.7% -2.3%, with high precision and accuracy. [Keywords:] heterocyclic amines, high performance liquid chromatography, atmospheric particles 1 Introduction Heterocyclic amines (Heterocyclic amines, HAs) are a class of meat, poultry, fish and other food processing generated when cooking with mutagenic and carcinogenic compounds [1]. The ratio of carcinogenic aflatoxin, several times higher nitrite amine, but also much higher than benzo (a) pyrene (BaP). Most of heterocyclic amines have been shown to cause multi-organ carcinogenesis experimental animals (such as liver cancer, colon cancer, lung cancer, breast cancer, skin cancer, oral cancer and stomach cancer, etc.) [2]. 70 foreign countries since the 20th century began to appear reports of heterocyclic amines, more concentrated in food [3 ~ 5], and isolated and identified from cooked foods in nearly 20 kinds of heterocyclic amines, but were very low (in ng / g total) [5]. [6 ~ 8], HAs also present in atmospheric pa