Dibutyl phthalate molecular mechanisms of male reproductive toxicity.docVIP

 PAGE \* MERGEFORMAT 10 Dibutyl phthalate molecular mechanisms of male reproductive toxicity Author: Ao Hong, LIN Ling, Kan Haidong, CHEN Bing-heng, Zhang Hui 【Abstract】 Objective To study the phthalate esters in rats the molecular mechanism of testicular toxicity. Methods RT-PCR to observe the dibutyl phthalate (DBP) on rat offspring at different developmental stages of testicular Mullerian inhibiting substance (MIS), androgen binding protein (ABP) and inhibin (inhibin ) expression levels of impact and use of ELISA to detect testosterone levels in rats after DBP exposure changes. Results Compared with control group, each rat testes exposed to DBP in the expression of MIS was no significant difference in serum total testosterone level was no significant difference, but high doses of DBP (1000mg/kg) group of Sertoli cells, ABP and inhibin (inhibin) of mRNA expression was significantly reduced, and with the testicular damage in a time - effect relationship. Conclusion environmental endocrine disrupting chemicals can interfere with DBP during the development of rat Sertoli cells, the expression of ABP and inhibin, its impact on the gonadal development of male reproductive toxicity of DBP may be the primary role of one of the mechanisms. Keywords: phthalate esters In recent years, plastic plasticizer dibutyl phthalate (DBP) is considered an environmental endocrine disruptors, mainly for the male reproductive and developmental toxicity (rats exposed to testicular atrophy, epididymal dysplasia, hypospadias , etc.) 〔1,2〕. In this study, phthalate DBP for (phthalates) the representations, observed during the development of DBP on the F1-generation rats testosterone (T) levels and androgen binding protein (Androgen-binding protein, ABP), inhibin (inhibin), mullerian inhibiting substance (MIS) gene expression, from the regulation of male reproductive system of the hypothalamus - pituitary - gonadal axis perspective of their reproductive and deve