Different doses of trichosanthin on Caski apoptosis.docVIP

 PAGE \* MERGEFORMAT 16 Different doses of trichosanthin on Caski apoptosis Of: Zhang Yanqiong AND MATERIA, Li Hongjun, Wu Jiangfeng [Abstract] Objective trichosanthin (TCS) on human cervical cancer cell growth inhibition and Caski dose - response relationship. Ways the role of different concentrations of TCS Caski in cultured human cervical carcinoma cell line, using acridine orange staining, fluorescence microscopy TCS Caski cells observed on the growth inhibition observed by transmission electron microscopy and TUNEL detection of apoptosis and TCS Caski Caski induced apoptosis dose-effect relationship. Results TCS on Caski cells significantly inhibited the growth, transmission electron microscopy and TUNEL France confirmed that the inhibition is achieved by inducing apoptosis. In a certain concentration range, number of apoptotic cells and the concentration of TCS between the dose dependent. Conclusion Caski TCS induced apoptosis in vitro, the best concentration 60 g / ml. [Keywords:] trichosanthin, Caski cells, apoptosis Abstract: ObjectiveTo study the inhibitory effect of different dose trichosanthin (TCS) on Caski cells. MethodsThe antiproliferative effect of different dose TCS on Caski cells was observed by fluorescence microscope. The TUNEL assay and electromicroscope were used for measurement of apoptosis of different dose TCS on The Caski. Results The apoptosis of different dose TCS on Caski Cells acted as the antiproliferation. The quantity of the apoptosis of the Caski cells depended on TCS ‘dose within certain range. ConclusionThe apoptosis of the Caski cells will happen when they were dealt with TCS and the best dose of the TCS is 60 g / ml. Keywords:: Trichosanthin; Caski cell; Apoptosis Cervical cancer is a common gynecological, a serious threat to women’s health. Trichosanthin (trichosanthin, TCS) from the Cucurbitaceae Trichosanthes Trichosanthin kirilouii Maxim’s roots to extract a single chain ribosome inactivating protei