Dispersed liquid-liquid microextraction rapid determination of furanocoumarins in Traditional Chinese Medicine compounds 4.docVIP

 PAGE \* MERGEFORMAT 16 Dispersed liquid-liquid microextraction rapid determination of furanocoumarins in Traditional Chinese Medicine compounds 4 Of: Zhang Yanjie Bai Xiaohong Li Lihua Wang Quande [Abstract] building distributed phase microextraction (DLLME combination with HPLC Determination of coumarins in traditional Chinese medicine samples furan chemical composition method. The factors affecting the extraction efficiency were optimized: containing 2.5% (w / V) NaCl The 1.5 mL sample solution by adding 50 μL CCl4 and 300 μL acetonitrile, distributed evenly to 3500 r / min centrifugation 3 min, drawing together CCl4 phase, 2 times the volume of methanol dissolved in CCl4 for HPLC analysis. psoralen , and oxidative imperatorin in the 0.006 ~ 6.00 mg / L range, imperatorin, isoimperatorin in the 0.006 ~ 12.0 mg / L range of good linearity, detection limit is 1.0 ~ 3.0 μg / L ( S / N = 3), the relative standard deviation of 2.3% ~ 5.4% (n = 5), concentration ratio of 12.6 ~ 38.5 times, 97.5% ~ 114.8% recovery. will this method for rapid determination of Angelica and its preparations per Central imperatorin Hu painkillers, isoimperatorin psoralen and trace elements, the content of oxidation imperatorin satisfactory results. [Keywords:] dispersed liquid-liquid micro extraction, HPLC, furan-type coumarins, Angelica 1 Introduction Umbelliferae Angelica is a plant whose active ingredient coumarin compounds with scattered wind and dampness, Tongqiao pain, swelling and abscess of the amplifier. Angelica coumarins in the furan-based high content of chemical components have imperatorin , isoimperatorin and so on, psoralen, etc. Imperatorin oxide content is low, not easy to determine. At present, the separation of coumarin compounds, extraction methods are solvent extraction, macroporous resin column chromatography, solvent extraction, etc., these methods are not only time consuming but also a large amount of organic solvent [1]. Determination of impera