Dorsal root ganglia primary sensory neurons of the temperature sensitivity of.docVIP

 PAGE \* MERGEFORMAT 5 Dorsal root ganglia primary sensory neurons of the temperature sensitivity of Authors: Zhang Lei, Wang Yuying, DONG Hui, Hu San-Jue [Keywords:] temperature-sensitive; patch-clamp technique; ganglia, spinal; neurons [Abstract] AIM: To study the effects of temperature on mediumdiameter primary sensory neurons in rat dorsal root ganglion (DRG). METHODS: Wholecell patchclamp recording was performed on mediumdiameter DRG neurons freshly isolated from rats for observing the firing characteristics by temperature gradient stimulation. RESULTS: According to the firing characteristics of the primary sensory medium neurons to the temperature changes in rat DRG, there were 2 classes of cells: In class Ⅰ , the medium neuron was temperaturesensitive neuron. Allornone action potentials could be generated and the number of spikes and the frequency decreased with increase of the temperature, and vice versa. The coldsensitive neuron was voltage dependent. In class Ⅱ , the medium neuron was temperatureinsensitive neuron. It only had single action potential with increase or decrease of the temperature, and the amplitude of the single action potential decreased with increase of the temperature. CONCLUSION: The two classes of rat DRG medium neurons have different thermosensitivity. [Keywords] thermosensitivity; patchclamp techniques; ganglia, spinal; neurons [Abstract] Objective: To observe the rat dorsal root ganglion (DRG) neurons of the temperature sensitivity of medium-sized. Methods: dispersed in fresh medium-sized rat dorsal root ganglion neurons using whole-cell patch-clamp technique to observe the temperature gradient given different stimuli caused by the characteristics of cell discharge. Results: According to medium-sized rat dorsal root ganglion neurons in the different temperature gradient stimulated discharge characteristics they can be divided into two types of temperature-sensitive neurons. Ⅰ type of temperature-sensitive neurons and cold