Dorsal vagal nucleus microinjection of somatostatin on the pancreatic activity of the neuronal.docVIP

 PAGE \* MERGEFORMAT 16 Dorsal vagal nucleus microinjection of somatostatin on the pancreatic activity of the neuronal Of: Xiao-Yan Chen, Cai Zhen Village, full Xiaohua, Wang Wei, Zhao-Shen Li [Abstract] Objective?: To observe the dorsal vagal nucleus (dorsal motor nucleus, DMN) microinjection of somatostatin (somatostatin, SS) neurons in rat pancreatic c-fos expression. METHODS: 250 ~ 300 g body weight 12 healthy male SD rats were randomly divided into two groups: experimental group (n = 6) DMN microinjection of 100 nL concentration 0.4g/mL the SS, the control group (n = 6) DMN microinjection of 100 nL artificial cerebrospinal fluid (aCSF). two lines utilized, are 20% fat emulsion infusion 1 h after duodenum, pancreas samples were obtained c-fos immunohistochemistry staining. Results: DMN SS rats injected with c-fos within the pancreas immunoreactive nerve fibers positive area (6? 122.5 + -1? 410.0 vs 1? 875.8 + -992.0) and the positive index (0.62 + -0.02 vs 0.26 + -0.07) were significantly lower (P? lt;0.01). Discussion: DMN microinjection of exogenous SS neurons in rat pancreas significant inhibitory effect, indicating that exogenous SS DMN neurons by inhibiting pancreatic exocrine pancreas to play on the inhibition. [Keywords:] dorsal vagal nucleus; somatostatin; pancreatic neurons; rat Abstract:? Objective: To observe the effect of microinjection of somatostatin? (SS)? Into the vagal dorsal motor nucleus? (DMN)? On the expression of c-fos in intrapancreatic neurons. Methods:? Twelve SD rats, weighing 250 ~ 300 g, were randomly selected and divided into two groups:? the experimental group and the control one.100 nL SS? (0.4g/mL)? was microinjected into the DMN in the experimental group (n = 6), while 100 nL artificial cerebrospinal fluid? (aCSF)? was microinjected into DMN in the control group (n = 6). One hour after the intraduodenal perfusion of 20% intralipid in each group, the rats’pancreases were obtained and studied by immunohistochemistr