Drug injection on the heat level in mice infected with influenza virus FM1 immune function of.docVIP

 PAGE \* MERGEFORMAT 13 Drug injection on the heat level in mice infected with influenza virus FM1 immune function of Study: King Shan, Gu Ligang Zhou Yun, Li Peng Tao [Abstract] Objective To study the drug injection on the heat level of influenza virus pneumonia in mice FM1 lung index, interleukin 4 (IL-4), interferon-γ (IFN-γ) gene transcription, CD40L expression levels and to explore the mechanisms of its anti-virus. Methods to ICR mice (SPF) for the study. were divided into 7 groups: injection drug hot flat major, medium and small dose group and the SHL group, ribavirin group, FM1 infected model group and normal group. FM1 virus infected mice after 7 d modeling continuous removal of lung tissue, lung index calculation, determined by RT-PCR in lung tissue INF-γmRNA, IL-4 mRNA gene transcription level, using Western blot, protein expression in lung tissue levels of CD40L. Results FM1 lung index infection model group was significantly higher than the normal group (P lt;0.001), ribavirin group, SHL Group, and large toxic heat level injection , medium and small dose group compared with the FM1 infection model, lung index were significantly lower (P lt;0.001 or P lt;0.01), which Yidu heat level in the dose group the best. toxin injection can reduce the heat level in mice infected with FM1 lung tissue IL-4 mRNA gene transcription (P lt;0.001), and the inverse relationship with concentration, but the heat level drug injection group IL-4 mRNA gene expression levels were significantly higher than that of ribavirin group (P lt;0.001 ), drug injection can enhance the heat level FM1 lungs of mice infected with IFN-γmRNA in gene transcription (P lt;0.001), and was positively correlated with the concentration, injection drug hot level, middle dose group and ribavirin group was not statistically significant (Pgt; 0.05). toxin injection can inhibit the heat level in mice infected lung tissue FM1 CD40L protein expression (P lt;0.001), inhibition was positively correlat