不同来源酶快速检测有机磷农药的灵敏度比较.pdfVIP

2008 年 9 月 农业机械学报 第 39 卷第 9 黯 不同来源酶快速检测有机磷农药的灵敏度比较赘 王小瑜卦霞王相友卦冬霞 【摘要 i 以商品乙酸胆碱黯爵〈来自家蝇)和自梢的鸡肝乙酸胆碱黯酶、鲤鱼脑乙酷胆碱酶离为研究对象，比 较 3 种不再浓度的有机磷农药{氧乐果、敌百虫和敌敌畏)对这 3 种酶的酶活抑制率。结果表明， 3 种农药对家蝇乙 酸胆碱醋酶的抑制率最高 E 对鸡肝和鲤鱼黯乙戳妞碱醋酶的酶活抑制率比家蝇低; 3 种乙戳Illi碱雷离对氧乐果都 不敏感。在检测精度要求不高的情况下可用鲤鱼脑酶作为敌敌畏的快速检测Jij酶，用鸡肝酶作为氧乐果的快速栓 费用酶。家蝇 AChE 是快速检测敌百虫的敏感酶。 关键理 z 农药残留 检测 乙雷在胆碱酶酶抑制率 中墨分类号: TS202.3 文献标识码 :A Sensitivity Comparison of Acetylcholinesterase from Different Sources in Rapid Detection of Organphosphorous Pesticide Residues Wang X?aoyu1 Sun Xìa1 Wang Xiangyou1 Sun Dongxia2 (1 . Shandong Unit洁白ty of Technology , Zibo 255049 ， α ina 2. Binzhou Instítute of Agricultural Machineη ， Binzhou 256616, China) Abstract The commercial acetylcholínesterase (AChE) from Musca domestica, and the acetylcholinesterase made by the researchers themselv，岱 from fowl liver and cyprinoids brain, were used for pesticides detεction. The inhibition of AChE activity under different ∞ncentratíons of omethoate, trichlorfon and d?chlorvos were ∞mpared.τhe results show that the inhibition of AChE activíty of Musca domestica is the highest of the three above. Three kinds of Acetylcholinesterase are not sensitive to omethoa饨. AChE from cyprinoids bra?n can be used for rapid detection of dichlorvos in the case of high detection precision is not needed. AChE from fowl liver can be used for rapid detection of trichlorfon. Key \Vords Pesticide residues, Detection, Acetylcholinesterase, Inhibition 碱醋酶结椅上存在一定的差异，对有机磷农药鸪拇 引言