EB virus BARF1 gene expression in gastric cancer cell line SGC7910 biological behavior.docVIP

 PAGE \* MERGEFORMAT 20 EB virus BARF1 gene expression in gastric cancer cell line SGC7910 biological behavior Of: Li Youqiong, Zhang Xueyi, Jian Chen Qiaolin Li Liangju was, as quiet, Zi Julimount, Zhou Ji [Abstract] Objective: To understand the EB virus BARF1 gene on biological behavior of gastric cancer cells. METHODS: pSG5/BARF1 eukaryotic expression vector, EB virus-negative transfected into gastric cancer cell lines SGC7901, by RT PCR identification, access to BARF1 stable expression cell clones. the pSG5 empty vector transfected cells as controls, the experimental induction of apoptosis, cell proliferation, soft agar colony formation assay and cell migration experiments. Results: Compared with control group, BARF1 expressing cells by group anticancer drug cisplatin induced apoptosis was significantly lower (P lt;0.01, proliferation significantly faster (P lt;0.01, soft agar colony formation was significantly increased (P lt;0.05, cell migration was significantly enhanced (P lt;0.05. Conclusion: BARF1 gene can fully enhance the immunogenicity of tumor cells of gastric cancer, gastric cancer may occur in the EB virus associated with the evolution play an important role throughout the process. [Keywords:] EB virus, BARF1, gastric cancer cells, cell biological behavior [Abstract] Objective: To investigate the oncogenic functions of EBV encoded BARF1 gene in gastric epithelial cells.Methods: A pSG5/BARF1 expression vector was constructed and transfected into an EBV negative and moderately differentiated gastric cancer cell line SGC7901.The stably transfected cell clones were generated by limiting dilution and G418 screening, and BARF1 expression was confirmed by RT PCR.The biological behavior analysis were conducted including cell survive curve drawing and apoptosis observation after Hoechst33342 staining induced by anti cancer drug diamminedichloroplatinum (DDP), cell growth curve drawing, soft agar colony formation assay, as well as cell migrati