Edaravone pretreatment in vitro neonatal rat brain cortex cells in cerebral ischemia-reperfusion injury in rats.docVIP

 PAGE \* MERGEFORMAT 9 Edaravone pretreatment in vitro neonatal rat brain cortex cells in cerebral ischemia-reperfusion injury in rats [Abstract] Objective: To study the effect of edaravone pretreatment in vitro neonatal rat brain cortex cells in cerebral ischemia - reperfusion injury in rats. Method: Remove Health within 24 h of newborn SD rat brain cortex cells in vitro to 7 days, were randomly divided into 5 groups: A group (normal control group); B group (drug injury group); C1 group (50 μ mol / L edaravone pretreatment group); C2 group (100 μ mol / L edaravone pretreatment group); C3 group (200 μ mol / L edaravone pretreatment group). C1, C2, C3 Group 7 days pretreatment with edaravone, 24 h after the B, C1, C2, C3 group was 200 μ mol / L glutamate 0.5 h, all groups continued to train for the normal culture medium 24 h observed after nerve cell survival (MTT), lactate dehydrogenase (LDH) leakage rate, apoptosis rate and HE staining under inverted phase contrast microscope, changes in cell morphology. Results: edaravone pretreatment MTT in each group different content than that in the injured group, LDH leakage volume and the percentage of apoptotic cells in varying degrees less than the injury group, under the inverted phase contrast microscope, see the pretreatment group compared with morphological damage of drug injury group, Light, 3 pretreatment group to C2, C3 group effect obvious, but no significant difference between the two groups. Conclusion: The use of the measured concentration of edaravone pretreatment 24 h earlier in vitro in young rats cerebral cortex cells, on cerebral ischemia - reperfusion injury has obvious protective effect. [Keywords:] edaravone; cerebral ischemia; ischemic preconditioning; apoptosis; lactate dehydrogenase; reperfusion injury [Abstract] Objective: To study the protective effect of edaravone pretreatment against cerebral ischemia-reperfusion caused injury in cultured cortical cells of suckling rat brain. Methods: Cort