Effect of glutamine on intestinal damage after hepatic inflow occlusion effect and mechanism of.docVIP

 PAGE \* MERGEFORMAT 14 Effect of glutamine on intestinal damage after hepatic inflow occlusion effect and mechanism of Of: Guo-Ping Liu, Wen Zhu Xi, Zhou Wenping, Cheng Guangming [Abstract] Objective To study glutamine (Gln after hepatic inflow occlusion on intestinal injury and its mechanism. Methods Male Wistar rats were 120, randomly divided into 3 groups: sham operation group (group 1, control group (2 group and the experimental group (3 groups. 40 .2 in each group and the 3 groups by Pringle’s method for hepatic inflow occlusion, sustained 35 minutes, in the 3 groups before occlusion in rats and Gln (300mg/kg , diluted with saline to 4ml, 2 times a day for 5 days. 10 rats in each group were randomly selected in the block before and 2,4,24 hours after reperfusion, intestinal tissue malondialdehyde determination (MDA content Serum levels of tumor necrosis factor-@ (TNF @ and portal vein endotoxin levels. light microscope and measured the thickness of intestinal mucosa, intestinal villus height. by reverse transcription polymerase chain reaction (RT PCR method detected 24 hours after reperfusion intestinal high mobility rate group protein B1 (HMGB1mRNA expression. Results Compared with group 1 after reperfusion group MDA levels 2,3, TNF @ and endotoxin levels increased (P lt;0.05, while the thickness of intestinal mucosa, intestinal villus height decreased (P lt; 0.05. and 2 group, 3 groups after reperfusion MDA content, TNF @ and endotoxin decreased (P lt;0.05, while the thickness of intestinal mucosa, intestinal villus height increased significantly (P lt;0.05. reperfusion after 24 hours, and 2 group, the expression levels of 1,3-group HMGB1mRNA decreased (P lt;0.05. Conclusions Gln can inhibit hepatic inflow occlusion after endotoxin translocation and intestinal HMGB1mRNA inflammatory cytokine expression, to relieve intestinal injury. [Keywords:] intestinal injury, glutamine, vascular occlusion, high mobility group protein B1 Abstract: Objecti