Effect of quercetin on corneal alkali burns scar formation.docVIP

 PAGE \* MERGEFORMAT 17 Effect of quercetin on corneal alkali burns scar formation Of: SOURCES, Qin Li, Zhang D, Han Caijuan [Abstract] Objective To investigate quercetin (quercetin, QU) in rat corneal alkali burns may inhibit the scar formation and its mechanism. Methods SD rat model of corneal alkali burn to his right eye as the experimental eye. SD rats randomly divided into 5 groups to the ointment matrix in control group, QU treatment group were given 2.5,5,10,20 g / kg QU eye ointment. Postoperative slit lamp examination, corneal opacity level recorded. 4 after operation , 7,14,21,28 days, animals were sacrificed and histological infiltration of inflammatory cells staining, Masson trichrome staining of corneal stroma and proliferation of the arrangement of collagen fibers, detected by immunohistochemistry of corneal tissue into growth factor-1 (TGF-1) and transforming growth factor- receptor (TGF-R ) expression. Results QU group pathological changes in the cornea and corneal opacity than in control group, 10g/kg QU and 20g/kg QU group inhibited the formation of corneal scar is stronger than 2.5g/kg QU and 5g/kg QU group, and 10g/kg QU and 20g/kg QU was no significant difference between the two groups. HE staining and Masson staining show QU Treatment of corneal stroma collagen fiber density than the control group. 7 days after alkali burn TGF-1 and TGF-R ‘s peaked, then gradually decreased to near normal levels, 10g/kg QU and 20g/kg QU group inhibition of TGF-1 and TGF-R expression was stronger than 2.5g/kg QU and 5g/kg QU group, and 10g/kg QU and 20g/kg QU was no significant difference between the two groups. Conclusion QU a certain degree of eye ointment reduce the role of scarring, particularly the concentration 10g/kg best. QU may inhibit TGF-1 and the expression of TGF-R play its role. [Keywords:] quercetin, corneal alkali burn, transforming growth factor-1, transforming growth factor- receptor ChinaABSTRACT: Objective To explore the