Effects of berberine on human nasopharyngeal carcinoma CNE2 content of intracellular calcium.docVIP

 PAGE \* MERGEFORMAT 6 Effects of berberine on human nasopharyngeal carcinoma CNE2 content of intracellular calcium Study: Biao Luo, Liang Jin, Huangshi Qing, Huang Bing, Houshuang Yan, Song-Feng Huang [Keywords:] Youjiang Affiliated Hospital of Medical Oncology, Baise, Guangxi 533000 Studies have shown that berberine on nasopharyngeal carcinoma cells significantly inhibited [1]. Mitochondria involved in tumor cell proliferation, apoptosis, the process of mitochondrial function and permeability of the mitochondrial membrane transmembrane potential difference close related. there experiments confirmed [2], intracellular calcium is stored in a large number of mitochondria, calcium ions in the induction of apoptosis play a major role. The role of berberine on cultured human nasopharyngeal carcinoma CNE 2 cells were CNE 2 levels of intracellular calcium ion concentration changes in nasopharyngeal carcinoma CNE 2 of berberine on the molecular mechanism of inhibition of tumor lines. Materials and methods 1. Drugs Berberine collected by the hospital itself, RPMI 1640 medium was Gibco BRL products, other reagents were of analytical grade. Calcium fluorescent indicator: Fluo 3/AM, Sigma, with dimethyl sulfoxide (DMSO) with into a 1.0 mM concentration. 2. Cell lines Human nasopharyngeal carcinoma CNE 2 cells were purchased from Cell Bank of Shanghai Institute of Life Sciences, the Court saved passage. 3. Methods (1) Cell culture: CNE 2 cells were routinely cultured with 10% fetal calf serum complete medium RPMI 1640 (penicillin 100 U / ml, streptomycin 100 U / ml, glutamic acid 30 g / ml at 37 , 5% CO2, saturated humidity in the culture, all experiments were carried out in the logarithmic phase cells. (2) growth inhibition assay [1]: logarithmic growth phase cells with complete culture medium to adjust cell number to 1 106 / ml, seeded in 96 well plates, 24 h later were added to a final concentration: 20 mg / L, 40 mg / L of berberine,