Effect of allicin on human gastric cancer SGC7901 cell growth.doc

 PAGE \* MERGEFORMAT 10 Effect of allicin on human gastric cancer SGC7901 cell growth [Abstract] Objective allicin (diallyl trisulfide, allicin) on human gastric cancer SGC7901 cell growth in vitro. Methods of allicin treatment of gastric cancer cell line SGC7901, MTT assay SGC7901 growth, apoptosis rate by flow cytometry, Immunocytochemical expression of Ki67 was detected. Results of allicin role in SGC7901 cells, their proliferation was inhibited showed a dose dependent manner; role of allicin in SGC7901 cells 12,24 and 48 h, cells apoptosis rates were 2.41%, 7.30% and 15.44%, significantly higher (P lt;0.05); the role of allicin after 48h, Ki67 positive expression rate decreased (P lt;0.05). Conclusion Allicin can inhibit gastric cancer SGC7901 cell proliferation and induce apoptosis. [Keywords:] allicin; gastric cancer; proliferation; apoptosis Abstract: Objective To investigate the effects of allicin on the proliferation and apoptosis of gastric cancer cell line SGC7901. Methods The SGC7901 cells were treated by allicin, and MTT assay was used to determine the cell growth. The apoptosis of SGC7901 was detected by flow cytometry (FCM). The expression of Ki67 in gastric cancer cells was detected by immunohistochemical staining. Results Allicin effectively inhibited the growth of SGC7901 gastric cancer cells in a concentration-dependent manner at a certain range of concentrations. The inhibitory rate was significantly higher than control group. 12h, 24h and 48h after allicin treatment, the apoptosis rate was 2.41%, 7.30% and 15.44%, respectively, with significantly higher effects as compared to the control group (P lt;0.05). The expression of Ki67 protein was down- regulated by allicin compared with the control group (P lt;0.05). Conclusion Allicin could inhibit the proliferation and induce apoptosis of gastric cancer SGC7901 cells. Keywords:: allicin; gastric tumor cell; proliferation; apoptosis Gastric cancer is one of the most common maligna