Effects of dexamethasone on LPS-induced acute lung injury in Rats.docVIP

 PAGE \* MERGEFORMAT 2 Effects of dexamethasone on LPS-induced acute lung injury in Rats [Abstract] Objective dexamethasone on LPS (Lipopolysaccharides, LPS)-induced acute lung injury when the impact of the role of inflammatory mediators to explore the protective mechanism of the lungs. Methods healthy male SD rats were randomly divided into A, B, C, D groups, each group respectively 1,2,4 h time point 3 to set up three sub-groups. A group by intraperitoneal injection of dexamethasone; B group by intraperitoneal injection of LPS, C group by intraperitoneal injection of LPS intraperitoneally 1h before the injection of dexamethasone, D group was the control group. After arrival at each time point, after intraperitoneal injection of double dose of thiopental death, tracheal, and I 0 ℃ 0.9% sodium chloride injection (normal saline, NS) for alveolar lavage. Adsorption using enzyme-linked immunosorbent assay (ELISA) determination of lavage fluid (broncho alveokar lavage fluid, BALF) in the tumor necrosis factor-α (tumor necrosis factor-α , TNF-α ), interleukin-1β (iaterlrukin-Iβ , IL-1β ) and macrophage inflammatory protein -2 (macrophafe inflamnatovy protein-2, MIP-2). Another 12 rats were randomly divided into groups according to the above four groups of three, ibid animal model for lung pathology observed. Pathological results with the D group, B group of serious lung injury. BALF of TNF-α , IL-1β , MIP-2 in the A, B, C, D between the two groups were statistically significant differences (F = 15.51,47.37,19.94,134.27,65.44,57.29,18.44,85.31, respectively, 89.13, P are