Emulsion solvent evaporationoxidation of liposome preparation.docVIP

 PAGE \* MERGEFORMAT 13 Emulsion solvent evaporationoxidation of liposome preparation [Abstract] Objective emulsion solvent evaporation method using liposomes prepared, and its Effect on water-soluble drugs oxymatrine encapsulation efficiency. Methods According to documents prepared by high shear mechanism has Oxymatrine water solubility W / O emulsion, and then the second phase of this emulsion emulsified water drops and continued rapid stirring, the solvent was evaporated to dryness. and the HPLC method using ultrafiltration entrapment efficiency of liposomes. Results of lipid emulsion prepared body encapsulation was low, less than 10% in most cases, when the hydration at high ionic strength solution at about 15% encapsulation efficiency, low temperature storage of samples after 2 weeks of drug crystal precipitation. Conclusion double emulsion method Oxidation of liposome encapsulation was lower, consistent with the reported cases. [Keywords:] liposomes; double emulsion solvent evaporation method; oxymatrine; soybean lecithin; encapsulation efficiency [Abstract] Objective To prepare liposomal oxymatrine by multiple emulsificating solvent evaporating process.Methods W / O emulsion that contained oxymatrine was prepared by employing high speed homogenizer.Liposomal oxymatrine was obtained by adding the W / O emulsion into aqueous media dropwise and stirring the mixture vigorously until the organic solvent was evaporated thoroughly.10 times higher of phospholipid concentration was used compared to that reported in literature.Ultrafiltration HPLC method is adopted to determine the encapsulation efficiency of oxymatrine liposome.Results The encapsulation efficiency was rather low by employing multiple emulsificating and solvent evaporating method that it was below 10% in most cases but some cases around 15%. Drug crystal could be found after the samples were stored 4 for 2 weeks.Conclusion The result is accordant with the reports in references that water