Epichlorohydrin for tissue engineering heart valve 1,3 in matrix metalloproteinase expression in.docVIP

 PAGE \* MERGEFORMAT 16 Epichlorohydrin for tissue engineering heart valve 1,3 in matrix metalloproteinase expression in Of: Wang Yun, Wei Xufeng, Guji Wei, Li Qing new Chen Yu, easy to set China [Abstract] Objective epichlorohydrin heart valve tissue engineering matrix metalloproteinase 1,3 (matrix metalloproteinase 1,3, MMP 1,3) rats. Methods Using detergents and trypsin digestion and prepared de- porcine aortic valve scaffold as a control group, with 30g / L 24h, epichlorohydrin scaffold to cells as the experimental group. The cultured human bone marrow mesenchymal stem cells (human bone marrow mesenchymal stem cells, hBMSCs) grown in the on the decellularized heart valve tissue engineering (tissue engineering heart valve, TEHV), paraffin-embedded sections were performed HE staining and scanning electron microscopy TEHV organizational structure, parallel RT PCR determination of the secretion of MMP 1,3 hBMSCs function. Results hBMSCs in the experimental group grew well on the surface of acellular valves, MMPs expression than the control group decreased. Conclusion epichlorohydrin treated acellular porcine aortic valve scaffold, can inhibit the expression of MMP 1,3 hBMSCs’s. [Keywords:] epichlorohydrin, matrix metalloproteinases, tissue engineered heart valve, degradation metabolic ABSTRACT: Objective To evaluate the effects of epoxy chloropropane on the expression of matrix metalloproteinase 1 and 3 in creating tissue engineering heart valves. Methods The porcine aortic valve leaflets were digested by using detergent and trypsin as control group, and those decellularized leaflets treated with 3 % epoxy chloropropan for 24 hours were the experimental group. The cultured human bone marrow mesenchymal stem cells (hBMSCs) were seeded onto the decellularized scaffolds of TEHV. The histological study was conducted with pathological sections and scanning electron microscopy. And reverse transcriptase polymerase chain reaction (RT PCR) was used to detec