Ephedrine Extraction Process.docVIP

 PAGE \* MERGEFORMAT 4 Ephedrine Extraction Process [Keywords:] ephedra ephedrine pseudoephedrine extraction process Ephedra Ephedra is the Ephedra family plant, wood thieves Ephedra and Ephedra in the quality of dry grass stems. With sweating solution table, Xuanfei asthma, diuretic effect, mainly used for cold cold, lung disease and without declaring the Chuankening prima facie evidence of edema treatment. Ephedra’s active ingredient is ephedrine and pseudoephedrine [1], in order to determine the active ingredient of ephedra the best extraction process, this study uses orthogonal to ephedrine content of the indexes, optimizing extraction process of the active ingredient of ephedra. An experimental material Cs  9301 dual-wavelength TLC scanner (Shimadzu, Japan); ephedrine hydrochloride reference substance, the Chinese pharmaceutical and biological products to provide for the determination of use; silica gel G (Qingdao Marine Chemical Plant); other reagents were analytically pure ; Ephedra (purchased from Jilin and Beijing Tong Ren Tang drugstore). 2 Methods and Results 2.1 The orthogonal design with the L9 (34) orthogonal to the content of ephedrine as an index of the solvent, extraction time, extraction frequency of visits in turn, set the level of form factors. 2.2 Preparation of taking ephedra extract medicinal herbs, said that taking an average of 9 copies, each 53 g, each one arranged according to the table plus 4 times the amount of solvent extraction, extract concentration and constant volume to 100 mL, which was. 2.3 Determination of ephedrine with the dual-wavelength TLC-scanning method. Thin-layer plate: 1.3% CMC  Na silica gel G plates; start agent: chloroform - toluene - methanol (3:1:1), in the chromatography tank placed a small cup of concentrated ammonia test solution, after the point-like silica gel G thin-layer chromatography plate into the tank pre-saturated 20 min and then started; reagent: 0.5% ninhydrin ethanol solution, the