ERCC1 protein expression and on the impact of oxaliplatin cytotoxicity.docVIP

 PAGE \* MERGEFORMAT 11 ERCC1 protein expression and on the impact of oxaliplatin cytotoxicity [Abstract] Objective To establish the expression of nucleotide excision repair cross complementary group 1 (ERCC1) protein in cell lines to evaluate ERCC1 expression of oxaliplatin cytotoxic effects. Methods extracted from human colorectal tumor tissues RNA, synthesis of cDNA, amplification of ERCC1 gene fragments, Gateway directional cloning transfected UV20 cells, cell inhibition rate measurement (SRB) Evaluation of oxaliplatin cytotoxicity. The results obtained successfully transfected UV20-ERCC1 cells, Western blot confirmed the expression of ERCC1 cell inhibition rate measurement (SRB) analysis shows that, ERCC1 expression was increased with reduced sensitivity to oxaliplatin are closely related. Conclusion Gateway can be successfully constructed with ERCC1 gene expression vector transfected cells, providing in vitro platform for ERCC1 gene function. Oxaliplatin cytotoxicity and ERCC1 gene expression level has a certain relevance. [Keywords:] cancer drug resistance In recent years, a new generation of platinum anticancer drug oxaliplatin has been effectively used in advanced colorectal cancer, lung cancer, ovarian cancer and other malignant tumors in the adjuvant chemotherapy. Cancer patients, however, the sensitivity of the individual pairs of oxaliplatin was very different, drug-resistant and sensitive person who compared survival times or 10 times less than 〔 1〕 . Nucleotide excision repair cross complementary group 1 (ERCC1) gene is a DNA damage repair pathway important gene. ERCC1 expression levels in tumor tissues and closely related to oxaliplatin resistance. In recent years, with the genetics of drug in-depth research, evaluation ERCC1 gene expression and single nucleotide polymorphisms (SNPs) and the platinum anticancer drugs associated with drug resistance, has been gradually attention 〔 2〕 . This study by Gateway cloning technology to create targe