ERCC1 gene silencing A2780 human ovarian cancer cell drug resistance of cisplatin.docVIP

 PAGE \* MERGEFORMAT 16 ERCC1 gene silencing A2780 human ovarian cancer cell drug resistance of cisplatin On: Sun Ye-Hong, William FK, Zhu Ying, Li Yuan body [Abstract] Objective: To study the RNA interference gene excision repair cross-complementing 1 (ERCC1) expression, the A2780 ovarian cancer cells resistant to cisplatin changes. Methods: A2780 cells were transfected with siRNA gene ERCC1 and GFPsiRNA, and to give smooth Platinum (to a final concentration of 3 mol / L), were detected by Western blot before and after cisplatin administration ERCC1 protein expression and cisplatin by MTT assay under different concentrations of A2780 cell viability. Results: ERCC1 siRNA transfection the experimental group of protein expression than the control group decreased significantly; ERCC1 siRNA + cisplatin group, ERCC1 expression was weaker than ERCC1 siRNA + PBS group, while GFP siRNA + cisplatin group protein is weaker than GFP siRNA + PBS group; cisplatin (concentration of 0.75 ~ 12 mol / L between) in a concentration dependent manner A2780 cell viability; in the same concentration under the action of cisplatin, compared with the GFPsiRNA, ERCC1siRNA group of cisplatin dose-response curve left. CONCLUSION: Cisplatin ERCC1 gene silencing can inhibit the growth of A2780 cells, and in a certain range of concentration in a concentration dependent manner; ERCC1 is involved in cisplatin resistance have one of the important factors. [Keywords:] RNA interference; excision repair cross-complementing gene 1; cisplatin; drug resistance; ovarian cancer [Abstract] Objective: To investigate the influence of RNA interfering the expression of excision repair cross complementing 1 (ERCC1) gene on the sensitivity of ovarian cancer cell A2780 to Cisplatin.Methods: SiRNA and GFP siRNA was transfected to knock out ERCC1 gene instantaneous and make a control manner respectively. Cisplatin (final concentration of 3mol / L) was given to the knockouted cells. Western blot wa