Fabrication of the anterior cerebral artery rat model of subarachnoid hemorrhage.docVIP

 PAGE \* MERGEFORMAT 10 Fabrication of the anterior cerebral artery rat model of subarachnoid hemorrhage [Abstract] Objective puncture produced subarachnoid hemorrhage (SAH) animal model for experimental study SAH provides simple, reliable method of modeling. Methods SD rats as experimental animals, the animals were divided into normal control group, sham operation group and model group. microsurgery isolated from rat left carotid artery, use the sharpen nylon thread through the external carotid artery into the carotid artery to the brain, the anterior cerebral artery puncture the formation of SAH. sham operation group, only isolated carotid artery puncture is not implemented. measured in each group 2 h, regional cerebral blood flow (rCBF), 3 d after the rats with DAB staining shows the amount of microvessel area, microvessel diameter measurement, density (MVD), microvessel area ratio (MVA). the results of model rat brain anatomy found large quantities of blood and subarachnoid blood clot, after 2 h, significantly lower rCBF, 3 d after the model rats frontal microvascular diameter, MVD, MVA decreased. Conclusion brain puncture before moving successfully established a simple, reliable, close to the clinical rat SAH model. [Keywords:] animal model of subarachnoid hemorrhage in the puncture SAH (Subarachnoid hemorrhage, SAH) is a common hemorrhagic cerebrovascular disease, usually secondary to aneurysm rupture, cerebral vascular accident living in the third, second only to cerebral thrombosis and hypertension bleeding, and its high mortality and morbidity [1,2]. to establish a simple, stable and reliable animal model of SAH to study the pathophysiological changes after SAH, the clinical treatment of great significance [3]. In this study, the anterior cerebral artery puncture technique in rats SAH model, by measuring regional cerebral blood flow (rCBF), microvessel diameter, density (MVD), microvessel area ratio (MVA) verify the reliability of the model fo