Failure of conventional chemotherapy in patients with tuberculosis 232 cases of TB drug resistance.docVIP

 PAGE \* MERGEFORMAT 8 Failure of conventional chemotherapy in patients with tuberculosis 232 cases of TB drug resistance Long-term use of anti-TB drugs for treatment of tuberculosis bacteria in patients with drug resistance, selected 232 cases of tuberculosis in patients with conventional chemotherapy is not valid sputum for culture and determination of bacterial resistance, as a guide for tuberculosis therapy. 1 Materials and methods 1.1 Case Selection select a city in February 1987 ~ August 1995 patients with conventional chemotherapy treatment of 232 cases invalid, 165 cases were male, female 67 cases, the age of 73 patients 30 years of age, 30 years old 49 years old in 121 cases, 50 years and over 38 cases, all patients hospitalized in the case of non-chemotherapy, the treatment with three drugs in 76 cases, two drug treatment, 86 cases more than 70 cases of isoniazid therapy as a single complex. 1.2 Experimental Methods 1.2.1 Specimens collected specimens from patients with deep before taking a daily early morning sputum for three consecutive days three times for inspection. 1.2.2 Direct smear cheese-like or granular material selected amount of smear to Kinyoun’s (1 acid-fast stain, microscopic examination. 1.2.3 sputum culture with 4% NaOH treatment for 25 minutes, and not by the centrifuge, take 0.1ml species in the LJ medium, inoculated with two of each specimen, 37 for 12 weeks incubation. 1.2.4 Determination of resistance to seven drugs were Department of crystalline powder, dilute the conventional method (2,3 its concentration (ug / ml were: isoniazid (INH1, 10,50,100,150,200,, streptomycin (SM10, 100 , 2000, on the ammonia acid (PAS1, 10,100, rifampicin (RFP50, 250,500,1000,1500,2000,3000, ethambutol (EB5, 50,100, kanamycin (KM1, 10,400, ammonia thiourea (TB110, 100. above were added to the LJ medium, 80 for one hour after the second gap sterilized for use. inoculum 10-2mg, 37 incubation for 4 weeks.