ezrin and merlin gene in pancreatic cancer cells and its significance.docVIP

 PAGE \* MERGEFORMAT 19 ezrin and merlin gene in pancreatic cancer cells and its significance [Abstract] Objective: To study the ezrin and merlin gene in pancreatic cancer cells and its effect on the biological behavior of pancreatic cancer cells. Methods: RT PCR and Western blotting, ezrin and merlin gene in pancreatic ductal cells and pancreatic cancer of the mRNA and protein levels; use of liposomes to ezrin and merlin gene were transfected into SW1990 cells, the application of MTT test, flow cytometry, migration assay and cell adhesion experiments observed gene transfection on cell proliferation , migration and adhesion, cell cycle and apoptosis. Results: ezrin and merlin gene in eight cells in different degrees, and its expression level and independent of the differentiation of pancreatic cancer cells. Compared with the control group, Gene transfection of ezrin and merlin slow SW1990 cells (P lt;0.01); cell cycle showed G1 phase cells increased, S phase cells decreased (all P lt;0.05); cell and matrix adhesion capacity decreased significantly (P lt;0.01 ); and the SW1990 cells transfected with merlin also significantly reduced their migration (P lt;0.05), and SW1990 cells transfected with ezrin in its migration was no statistically significant change (Pgt; 0.05). Conclusion: ezrin and merlin protein in pancreatic cancer cell proliferation, progression may play a negative regulatory role. [Keywords:] pancreatic cancer; ezrin gene; merlin gene; proliferation; migration Ezra ezrin protein is protein, protein and membrane protrusion root protein (ezrin radixin moesin, ERM) family member, and the merlin protein belong to protein 4.1 superfamily. human ERM, 75% between 80% homology human merlin and the ERM protein only 45% homology [1]. ezrin and merlin protein was mainly localized in the cytoskeleton remodeling of the region, such as the cell membrane folds, dynamic actin filaments, and other parts of the cleavage furrow, mainly in epithelial cells bet