Fang Zhou Ke Kim Yan A549 lung cancer cells resistant component analysis of effective part.docVIP

 PAGE \* MERGEFORMAT 11 Fang Zhou Ke Kim Yan A549 lung cancer cells resistant component analysis of effective part Of: Dong Jing, Yin Lin, Zhang Xu, Duan Jin Ao [Abstract] Objective of Zhou Fang Ke Kim Yan effective part Ⅰ qualitative and quantitative analysis of the composition, for in-depth study on Anti Zhou grams of gold rock material basis for effective fractions of lung cancer foundation. Methods Qualitative methods in vitro reaction and TLC identification of effective component parts, the establishment of sodium nitrite - nitrate Determination of total flavonoid content of aluminum, vanillin - acetic acid - perchloric acid colorimetric determination of total saponins. Results Zhou Fang Ke Kim Yan main containing effective part Ⅰ flavonoids, saponins, the content was 0.85%, 1.07%, purity 29.7%, 37.4%. Conclusion Zhou Fang Ke Kim Yan Ⅰ in the active fraction containing mainly flavonoids and saponins, with a total purity of 67.1%. [Keywords:] Fang Zhou Ke Kim Yan, qualitative identification, flavonoids, saponins Abstract: Objective To study material basis of Zhou’s prescription, components of effective parts Ⅰ in Zhou’s prescription was gualitative and quantitative analyzed. Methods The components of effective part Ⅰ was identified by test-tube reaction and qualitative TLC method, the content of saponins and flavones was quantitative analyzed. Results Total content of the saponins and flavone are 1.07% and 0.85% in Zhou’s Prescription. The purity of saponins and flavones in effective parts Ⅰ are 37.4% and 29.7%. Conclusion The main components of effective part Ⅰ in Zhou’s Prescription were flavones and saponins. Key word: Zhou’s prescription, qualitative identification, saponins, flavones Ke Kim Yan Zhou Fang was a famous Chinese professor Zhouzhongying clinical treatment of lung cancer experience side, mainly by Radix, Radix, Houttuynia, safflower and other components. Zhou grams of gold rock side of the effective parts of the system eval