FL-1 macroporous resin separation and purification of total flavonoids of leaves of guava_0.docVIP

 PAGE \* MERGEFORMAT 10 FL-1 macroporous resin separation and purification of total flavonoids of leaves of guava Of: Chengbing Jie Zhou Ying-chun Liu Qiang Huang Haijun Yan Wen Chen Baotian [Abstract]: Objective To investigate the FL-1 macroporous resin purification of total flavonoids of guava leaf conditions and parameters. Methods Determination of total flavonoids content of guava leaf, resin adsorption, the volume of the fluid volume Indexes Inspection guava FL-1 resin adsorption of total flavonoids. Results FL-1 macroporous resin adsorption and purification of total flavonoids Psidium the best conditions were: sample concentration of total flavonoids 13.22%, crude drugs and cast resin ratio was 1:4, with 70% ethanol as eluent, elution flow rate of 2 mL / min. Using this technology, guava leaf flavonoids of the yield reached 30.12%, 55.22% purity. Conclusions The process is simple, guava leaves for purification of total flavonoids absorption. [Keywords:] guava leaf flavonoids macroporous resin Abstract: Objective To evaluate the performance of FL-1 macroporous absorption resin for absorption of total flavonoids from Psidium guajava leaf. Methods The concentration of total flavones in Psidium guajava leaf was determined by ultraviolet spectrophotometry and the absorption behavior of FL-1 macroporous adsorption resins to total flavones in Psidium guajava leaf was examined for the adsorption capacity and the volume of solution loaded. Results Optimal absorption of total flavonoids was achieved with the sample: total flavonoids concentration in the solution of 13.22 mg / mL, the ratio of total flavonoids concentration and macroporous adsorption resin was 1:4, washing with 70% ethanol at the flow velocity of 2 BV / h. Conclusion FL-1 Macroporous absorption resin can be well applicable for enrichment of total flavonoids in Psidium guaijava leaf. Keywords:: Psidium guajava leaf, total flavonoids; macroporous adsorption resins Guava (Psidium gua