High mobility group protein B1 myocardial ischemia reperfusion injury in Progress.docVIP

 PAGE \* MERGEFORMAT 20 High mobility group protein B1 myocardial ischemia reperfusion injury in Progress [Keywords:] myocardial reperfusion injury; HMGB1; inflammatory reaction; myocardial regeneration High mobility group protein (HMG), a result of polyacrylamide gel electrophoresis in (PAGE) in a high migration named, is a class of widely distributed in the nucleus of higher eukaryotic DNA binding proteins of non group, is to maintain nucleosome stability control, DNA replication, recombination, transcription and other processes is not the lack of regulatory proteins. HMGB1 member of his family in cell death or injury, release into the extracellular or serum have widespread effects of cell biology, In many inflammatory diseases play an important role in the pathogenesis. In recent years found that, HMGB1 in myocardial ischemia and reperfusion injury play an important role in the mechanism. 1HMGB-1 of the structure and distribution HMGB1 is a large number of eukaryotic cells the existence of a chromosome-binding protein, and its gene mapped to human chromosome 13q12 band, containing 5 exons, the sequence is highly conserved. HMGB1 molecular weight of about 25 kD, 219 amino acid residues from the composed of the sequence in the evolutionary process is highly conserved in all mammals in, HMGB1 homology of 99%. its molecular structure containing domains with DNA binding A box (aa1 ~ 79) and B boxes (aa89 ~ 163) and a highly repetitive and rich in acidic amino acid C-terminal (aa186 ~ 215). which is the HMGB1 B box induced cytokine production in part of its active site in the first 20 amino acid residues (1), recombinant B-box to simulate the full-length protein molecules, monocytes / macrophages and neutrophils secrete TNF-@ and other proinflammatory cytokines. The recombinant A box can antagonize the full-length protein molecules and recombinant B box protein induced inflammation. HMGB1 is widely distributed in lymphoid tissues, brain, liver, lung, h