HPLC Determination of Amoxicillin and Dicloxacillin sodium capsules.docVIP

 PAGE \* MERGEFORMAT 12 HPLC Determination of Amoxicillin and Dicloxacillin sodium capsules [Abstract] Objective To establish the amoxicillin Dicloxacillin Sodium Capsules Dicloxacillin sodium amoxicillin and method for the determination. Methods Shimadzu VP  ODS column, mobile phase 0.008 mol / L sodium dodecyl sulfate (adjusted with phosphoric acid to pH4.5)  methanol (volume ratio 68:32), detection wavelength 225 nm. The results of Amoxicillin and Dicloxacillin in the chromatographic conditions, have the right to retain, measured at 10 min to complete. Amoxicillin at 0.0050 ~ 1.0000 mg / mL, dicloxacillin in 0.0025 ~ 0.5036 mg / mL concentration and peak area within the linear relationship. Amoxicillin and Dicloxacillin sodium average recoveries (n = 9) were 99.4% (RSD = 1.0%) and 99.1% (RSD = 0.97%). Conclusion The method is accurate, reproducible, high sensitivity, can be used for the product quality control. [Keywords:] Amoxicillin Sodium Capsules Dicloxacillin Dicloxacillin high-performance liquid chromatography amoxicillin Abstract: Objective To establish an HPLC method for simultaneously assaying of amoxicillin and dicloxacillin in capsules.Methods The separation was performed on a Shimadzu VP  ODS column. The mobile phase was 0.008 mol / L sodium lauryl sulpate (adjusted with phosphoric acid to pH4. 5)  methanol (68:32). The detection wavelength was 230 nm. Results Amoxicillin and dicloxacillin had suitable retention time, which could be separated within 10 min. The standard curves of amoxicillin and dicloxacillin were all linear over the concentrations of 0.0050 ~ 1.0000 mg / mL (r = 0.9999, n = 7) and 0.0025-0.5036 mg / mL (r = 0.999 9, n = 7), respectively. The average recoveries of amoxicillin and diclxacillin were 99.4% (RSD 1.0%) and 99.1 % (RSD 0.97%), respectively. Conclusion This method is simple, sensitive, accurate and reproducible, which can be used for the quality of amoxicillin  dicloxacillin capsule. Keywords:: amoxicillin