HPLC Determination of Guiqi Zhuanggu granules stilbene glucoside content of.docVIP

 PAGE \* MERGEFORMAT 14 HPLC Determination of Guiqi Zhuanggu granules stilbene glucoside content of Author: Gao Yong Tang 1, Yi Xue-Long 1, Wang Zhao-Hua 2 【Abstract】 Objective high-performance liquid chromatography Guiqi Zhuanggu granules stilbene glucoside content. Methods DiamonsilTM (diamonds) C18 (5μm, 250mm × 4.6mm), acetonitrile -0.2% phosphoric acid (seventy-seven past eleven p.m.) as mobile phase, flow rate: 1ml/min, detection wavelength 320nm. The results of the linear range of 0.0964 ~ 0.482μg, r = 0.9998, average recovery was 98.00%, RDS = 0.98%. Conclusions The method used to determine the Guiqi Zhuanggu granules stilbene glucoside content, simple, rapid and accurate. Keywords: Guiqi Zhuanggu particle; stilbene glycoside; high-performance liquid chromatography 【Abstract】 Objective To develop a HPLC method to determine the content of 2,3,5,4 ‘-tetrahydroxystibene-2-o-β-D-glucoside in Guiqizhuanggu granule.Methods The HPLC method was carried out on an DiamonsilTMC18 column ( 5μm, 250mm × 4.6mm) using a mobile phase of acetonitrile-0.2% phosphoric acid water (23:77), and detection wavelength was 320 nm.Results With a relation efficient of 0.9998. Good linear relationshipe between area and a mount was noted for 0.0964 ~ 0.482μg. The average recovery of 2,3,5,4 ‘-tetrahydroxystibene-2-o-β-D-glucoside and RSD were 98.00% and 0.98% respectively. Conclusion It was proved that the method was available, simple and accurate. 【Keywords:】 guiqizhuanggu granule; 2,3,5,4 ‘-tetrahydroxystibene-2-o-β-D-glucoside; HPLC Guiqi Zhuanggu particles are Rehmannia glutinosa and shells, the composition of Eucommia and other 18 Chinese herbal medicines. Main function of qi tonic, nourishing the liver and kidney, gluten strong bone. Applicable to Yaoxisuanruan, insufficient blood embolism. Fang major drug Polygonum multiflorum Thunb dry roots. One of its active ingredients stilbene glucoside. In this study, using high perf