HPLC Determination of heat Runtong capsule contents of emodin and Chrysophanol.docVIP

 PAGE \* MERGEFORMAT 9 HPLC Determination of heat Runtong capsule contents of emodin and Chrysophanol [Abstract] Objective: To determine the heat Runtong capsule contents of emodin and Chrysophanol. Methods: HPLC method, column for the YWG C18 column (250 mm * 4.6 mm, 5 μ m); methanol -0.1% phosphoric acid (85:15) as mobile phase; column temperature was 25 ℃ , detection wavelength of 254 nm The flow rate was 1.0 mL / min. Results: The linear range of emodin 22.9 μ g ~ 183.2 μ g, chrysophanol linear range of 20.1 μ g ~ 160.8 μ g; average recoveries, emodin for 97.92%, RSD = 1.75% (n = 5), chrysophanol of 97.62%, RSD = 1.18% (n = 5). Conclusion: The method is simple, rapid, accurate, and can be used to heat Runtong capsule quality control. [Keywords:] heat-Run capsules Chrysophanol HPLC Determination of emodin Abstract Objective: To establish a HPLC method for determination the contents of emodin and chrysophanol in Qingre Runtong capsule. Methods: HPLC was performed on YWG-C18 (250 mm * 4.6 mm, 10 μ m) column with methanol-0.1% phosphoric acid (85 : 15) as mobile phase at flow rate of 1.0 mL / min. The column temperature was 25 ℃ and the detection wavelength was 254 nm. Results: The liner ranges of emodin and chrysophanol were 22.9 μ g ~ 183.2 μ g and 20.1 μ g ~ 160.8 μ g . The average recovery rate of emodin was 97.92% (RSD = 1.75%, n = 5) and that of chrysophanol was 97.62% (RSD = 1.18%, n = 5). Conclusion: This method is simple, quick, accurate, which can be used for the quality control of Qingre Runtong capsule. Key word Qiangre Runtong capsule; emodin; chrysophanol; HPLC; quantitative determination Run heat Capsule is rhubarb, Cortex Moutan, angelica, citrus aurantium and many other Chinese herbal medicines made of compound preparation, with heat dehumidification, Runchang laxative effect, clinically for the treatment of large intestine damp-heat-induced constipation, gingival swelling, pain, sore tongue, etc. Syndrome. The preparation process in th