HPLC Determination of Forsythia sepsis pills contents of emodin and chrysophanol.docVIP

 PAGE \* MERGEFORMAT 13 HPLC Determination of Forsythia sepsis pills contents of emodin and chrysophanol 【Abstract】 Objective: To establish Forsythia sepsis pill method for the determination. Methods: The high-performance liquid chromatography. Column: SHIMADZU VP-ODS (250 mm × 4.6 mm, 5 um), mobile phase: methanol -0.1% phosphoric acid (80:20, V / V), flow rate: 1.0ml * min-1, detection wavelength : 254nm, column temperature: 30 ℃. RESULTS: Emodin in 10.13 ~ 250.8μg * ml-1 range of a good linear relationship (r = 0.9996); The average recovery was 96.2%, RSD = 1.2% (n = 6); rhubarb phenols 10.00 ~ 250.0μg * ml-1 range a good linear relationship (r = 0.9998); The average recovery was 96.7%, RSD = 1.3% (n = 6). Conclusion: This method is simple, accurate and reliable, suitable for quality control pills Forsythia sepsis. Keywords: high-performance liquid chromatography forsythia sepsis pill Chrysophanol Determination of Emodin Determination the content of Emodin and Chrysophanol in Lianqiao baidu Pills by HPLC LI Ya-rong Wang-Shu (Jilin Institute for Food and Drug Control Jilin Jilin 132001) 【Abstract objective】 To establish a HPLC method for determination the content of emodin and chysophanol in. Lianqiao baidu Pills METHOD: The shimadzu VP-ODS C18 (250 mm × 4.6 mm, 5 um) was used, the mobile phase was methanol-0.1 % phosphoric acid solution (80:20), the column temperature was 30 ℃, the detection wavelength was 254 nm.the flow rate was 1.0ml * min-1. RESULTS The linear ranges of emodin and chrysophanol were at 10.13 ~ 250.8μg * ml-1 (r = 0.9996) and10.00 ~ 250.0μg * ml-1 (r = 0.9998) respectively, the average recoveries (n = 6) were 96.2% and 96.7% with RSD 1.2% and 1.3% respeclivery. CONCLUSION The method is simple and accurate, it can be used for quality control of lianqiao baidu Pills. 【Keywords:】 HPLC; lianqiao baidu Pills; emodin; chrysophanol; determination Forsythia sepsis pill as “the Ministry of Healt