HPLC Determination of intestinal Ning Capsules Isopsoralen content.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of intestinal Ning Capsules Isopsoralen content [Abstract] Objective: To establish intestinal Ning Capsule Assay. METHODS: HPLC method. Column: Kromasil C18 column, mobile phase: methanol - water (60:40), detection wavelength: 245nm, column temperature: 40 ℃ , flow: 0.81ml/min. Results: Isopsoralen sample volume of 0.026 ~ 0.312μ g within the sample volume and the peak area showed good linear relationship (r = 0.9998); The average recovery was 98.6529%, RSD 1.43% ( n = 6). Conclusion: This method is simple and easy, the agency is strong, accurate and reliable, and can be used for intestinal Ning Capsule quality control. [Keywords:] high-performance liquid chromatography; intestinal Ning Capsules / chemical synthesis; isopsoralen / Standard Intestinal Ning Capsule is my correction in research and development of a new drug, it is by psoralen (salt) 160g, Atractylodes macrocephala (fried) 160g, Chinese yam (fried) 120g, Poria 120g, gallic 80g, Qin Paper 80g, nutmeg ( baked) 80g, costas (baked) 40g, Evodia 40g, Baked Licorice 40g composed of 10 Herbs compound preparation, can be used for the treatment of chronic non-specific ulcerative colitis to good effect. Using high performance liquid chromatography on intestinal Ning Capsule in Isopsoralen for determination to control its quality, so that the efficacy between the batches of preparation and stability, are reported below. 1 Materials Waters 600E dual pump head plunger reciprocating pump, Waters 2487 dual wavelength UV detector, Chromstation data processing system. Isopsoralen reference substance (China Pharmaceutical and Biological Products Institution, for the determination of use, Lot :0738-200108); intestinal Ning Capsule (self-control, batch number:20050314; lack of psoralen in negative samples (self); methanol as the chromatography of pure water as Wahaha purified water, and other reagents were of analytical grade. 2 Methods and Results