HPLC fingerprint of Radix Yangjinhua_0.docVIP

 PAGE \* MERGEFORMAT 10 HPLC fingerprint of Radix Yangjinhua [Abstract] Objective To establish Yangjinhua HPLC fingerprint of herbal medicine. Methods Zorbarx C18 (4.6 mm × 250 mm, 5 μm) column was used, 0.07 mol / L sodium phosphate solution (pH 6.0 adjusted with phosphoric acid , containing 17.5 mmol sodium lauryl sulfate) - acetonitrile (2:1) as mobile phase, flow rate 0.8 mL / min, detection wavelength 216 nm. The results of this method reproducibility and precision were good, cluster analysis showed that Southeast Asia Golden and Golden Northern significantly different chemical composition. Conclusion The medicine can effectively control the quality of Yangjinhua. [Keywords:] Yangjinhua, fingerprints, scopolamine hydrobromide, HPLC Keywords:: Flos Daturae, fingerprint, scopolamine, HPLC 2005 Edition lt;lt;Chinese Pharmacopoeiagt; “(a) gathers Yangjinhua for the Solanaceae plant Datura metel L. Datura dry flowers. In addition, the local administration is also Solanaceae Datura (Datura) Mandala D.innoxia hair flower plants, the former known as the “South Sea Golden Flower”, which is called “Northern Golden Flower.” Yangjinhua is a drama poison chemicals, with asthma cough, analgesic, antispasmodic effect, for asthma, cough, abdominal Leng Tong, and surgical anesthesia, Fengshibitong. So far, Yangjinhua has been isolated and identified 39 kinds of alkaloids and 13 lactone components, including main active ingredients of scopolamine and hyoscyamine. At present, multi-use titration [1], TLC [2], gas chromatography and high performance liquid chromatography (HPLC) [3 - 4] the analysis of one of these alkaloids, but can not effectively control the intrinsic quality of medicine. In this experiment, 10 batches of different sources investigated Yangjinhua herbs (including the Golden South Seas, the Northern Golden Flower), the establishment of a fingerprint, and Determination of different species Yangjinhua the cluster analysis results. An instrument