Human parvovirus B19 infection associated with Kikuchi#39;s disease.docVIP

 PAGE \* MERGEFORMAT 9 Human parvovirus B19 infection associated with Kikuchi#39;s disease 【Key Words】 Virus Relationship between human parvovirus B19 infection and KikuchiFujimoto disease 【Abstract】 AIM: To explore the relationship between parvovirus B19 infection and idiopathic KikuchiFujimoto disease (KFD). METHODS: Tissue blocks from lymph nodes of 32 idiopathic KFD patients and from normal lymph nodes of 13 normal controls were detected for parvovirus B19 DNA and viral proteins by in situ hybridization and immunohistochemical techniques in a doubleblind manner. In situ hybridization and immunohistochemical doublestaining were used to determine the type of virusinfected cells. RESULTS: In situ hybridization and immunohistochemistry revealed that the positive rates of B19 DNA and viral proteins were 71.9% and 59.4% respectively in the 32 idiopathic KFD patients. In the control group, the positive rates were 30.8% and 23.1%, respectively. Statistical analysis indicated that the positive rates of B19 DNA and proteins differed significantly between the idiopathic KFD patients and the controls (P = 0.011 and P = 0.027 respectively). The B19 virusinfected cells were mainly lymphocytes and a small number of histocytes. CONCLUSION: Lymph nodes from idiopathic KFD patients have higher parvovirus B19 infection rates than those from controls. Parvovirus B19 infection may play an important role in the pathogenesis of idiopathic KFD. 【Keywords】 histiocytic necrotizing lymphadenitis; parvovirus B19; in situ hybridization; immunohistochemistry 【Abstract】 Objective: To study the human parvovirus B19 infection and histiocytic necrotizing lymphadenitis (KFD) correlation. Methods: The double-blind method in 32 cases of KFD patients and 13 normal controls lymph node biopsies using in situ hybridization and immunohistochemical staining to detect B19 virus genome DNA and viral proteins. and using in situ hybridization and immunohistochemistry double-